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e2f2 polyclonal antibody  (Thermo Fisher)


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    Thermo Fisher e2f2 polyclonal antibody
    E2f2 Polyclonal Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/e2f2+polyclonal+antibody/10__1016_slash_j__hlife__2024__12__005-224-130-133?v=Thermo+Fisher
    Average 90 stars, based on 1 article reviews
    e2f2 polyclonal antibody - by Bioz Stars, 2026-07
    90/100 stars

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    <t>E2F2</t> is a direct target of miR-218. (A) Predicted binding sites for the seed sequences of miR-218 and miR-520a in the E2F2 3′-UTR and the sites of target mutagenesis are presented. (B) A dual luciferase assay was conducted in order to confirm the direct regulation of miR-218 on the E2F2 3′-UTR. miR-218 and miR-520a regulate E2F2 expression at the (C) mRNA and (D) protein levels in Huh7 and MHCC-97H cells. Values are presented as the mean ± standard deviation (n=3). * P<0.05, ** P<0.01, vs. NC. miR-218, microRNA-218; UTR, untranslated region; NC, negative control; Wt, wild-type; mt, mutant.
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    <t>E2F2</t> is a direct target of miR-218. (A) Predicted binding sites for the seed sequences of miR-218 and miR-520a in the E2F2 3′-UTR and the sites of target mutagenesis are presented. (B) A dual luciferase assay was conducted in order to confirm the direct regulation of miR-218 on the E2F2 3′-UTR. miR-218 and miR-520a regulate E2F2 expression at the (C) mRNA and (D) protein levels in Huh7 and MHCC-97H cells. Values are presented as the mean ± standard deviation (n=3). * P<0.05, ** P<0.01, vs. NC. miR-218, microRNA-218; UTR, untranslated region; NC, negative control; Wt, wild-type; mt, mutant.
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    E2F2 is a direct target of miR-218. (A) Predicted binding sites for the seed sequences of miR-218 and miR-520a in the E2F2 3′-UTR and the sites of target mutagenesis are presented. (B) A dual luciferase assay was conducted in order to confirm the direct regulation of miR-218 on the E2F2 3′-UTR. miR-218 and miR-520a regulate E2F2 expression at the (C) mRNA and (D) protein levels in Huh7 and MHCC-97H cells. Values are presented as the mean ± standard deviation (n=3). * P<0.05, ** P<0.01, vs. NC. miR-218, microRNA-218; UTR, untranslated region; NC, negative control; Wt, wild-type; mt, mutant.

    Journal: Molecular Medicine Reports

    Article Title: MicroRNA-218 and microRNA-520a inhibit cell proliferation by downregulating E2F2 in hepatocellular carcinoma

    doi: 10.3892/mmr.2015.3516

    Figure Lengend Snippet: E2F2 is a direct target of miR-218. (A) Predicted binding sites for the seed sequences of miR-218 and miR-520a in the E2F2 3′-UTR and the sites of target mutagenesis are presented. (B) A dual luciferase assay was conducted in order to confirm the direct regulation of miR-218 on the E2F2 3′-UTR. miR-218 and miR-520a regulate E2F2 expression at the (C) mRNA and (D) protein levels in Huh7 and MHCC-97H cells. Values are presented as the mean ± standard deviation (n=3). * P<0.05, ** P<0.01, vs. NC. miR-218, microRNA-218; UTR, untranslated region; NC, negative control; Wt, wild-type; mt, mutant.

    Article Snippet: Following blocking with 5% skimmed milk, the membranes were incubated with rabbit anti-human E2F2 polyclonal antibody (1:200; sc-632; Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA) overnight at 4°C.

    Techniques: Binding Assay, Mutagenesis, Luciferase, Expressing, Standard Deviation, Negative Control